SDS-PAGE explained - Protein Separation Technique
25 ml, ≥98% pure 2-mercaptoethanol. 2-Mercaptoethanol, >=% · Application. BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually. 2-Mercaptoethanol solution has a concentration of approx. M. Quality Level vapor density (vs air) vapor pressure 1 mmHg (20 °C) assay ≥
Preparing Tris Buffer
Technical Details ; Vapor Pressure, 1 mmHg (20 °C) ; Assay, ≥% ; Form, liquid ; Expl. Lim. 18 % ; Concentration, M (pure liquid). molecular mass which is given on the bottle there is no other indication of what the concentration of 2-mercaptoethanol in the bottle is. Please can anyone. Always use inside of a chemical fume hood. 2-Mercaptoethanol has an unpleasant odor at extremely low concentrations. Therefore, extreme care should be taken.
2-Mercaptoethanol ≥98% ; Formula: HSCH₂CH₂OH ; MW: g/mol ; MDL Number: MFCD ; CAS Number: ; UN: 2-Mercaptoethanol ; Odor, Disagreeable, distinctive ; Density, g/cm ; Melting point, − °C (− °F; K) ; Boiling point, °C; °F; K. 2-Mercaptoethanol(x) is provided at a concentration of 55 mM in Dulbecco's Phosphate Buffered Saline (DPBS).
Many protocols add b-mercaptoethanol to cell media (concentrations around mM) in order to keep T cells, macrophages, and DC cultures viable; however, some. Stock concentration = 5x M. To use: Add 1 μl/ml media. Final concentration = 5x M. 5% B-mercapto is optimal and ensures complete reduction in disulfide bridge. % should also be fine As Andreas said, you can always supplement it to 5%.
g/mL at 25° C (lit.) Refractive Index: n20 D (lit.) pK Values. 2-Mercaptoethanol ; As low as ₹ ; Availability: In stock ; SKU: MB 2-Mercaptoethanol (2-ME) is a clear colorless to very faint yellow liquid that boils at – °C and has a concentration of M (mol l−1). The bulk. May be toxic by ingestion, inhalation, or skin absorption.|Mercaptoethanol is a primary alcohol and an alkanethiol. It has a role as a geroprotector.|A water-.